Pine wood nematode detector

1、Pine wood nematode detector Instrument Application

Pine wood nematode detector is based on a real-time quantitative PCR method，It only requires lysing the nematode sample, and the resulting lysate can be used directly for the identification of pine wood nematodes.Pine wood nematode detector system primarily involves the isolation of pine wood nematodes, DNA extraction, and fluorescence quantitative PCR detection. It can quickly complete the detection of pine wood nematodes, automatically display the detection results and print detection reports, eliminating the need for manual interpretation. With an accuracy rate greater than 99%, it greatly improves the efficiency of pine wood nematode detection.  The system can be configured with a portable field testing system for mobile operation, providing convenience for field detection of pine wood nematodes. Pine wood nematode detector is widely used in forestry bureaus, forest protection stations, quarantine bureaus, customs, universities, research institutions, and other organizations.

2、Pine wood nematode detector Instrument Features:

1. High system detection accuracy.

2. The intelligent pine wood nematode DNA rapid detection system has a built-in automated pine wood nematode DNA detection program, eliminating the need for complex program settings.  It operates automatically with a single click and can detect not only nematode separation liquid but also pine wood chips directly without prior nematode separation.

3. The system is easy to operate, saving time and increasing efficiency, making it suitable for forestry and plant protection departments at all levels. The entire process, including sample processing, DNA extraction, instrument detection, and result interpretation, can be completed in as little as 60 minutes.

4. Utilizing amplification fluorescence technology, the system's main unit can fulfill laboratory detection and identification requirements.

5. Small size and lightweight, making it easy to carry and ideal for field experiments.

6. Built-in 10-inch high-definition capacitive touchscreen PDA for simple and quick operation.

7.  Features a high-quality Marlow Peltier cooling element, combined with a high-end German PT1000 temperature sensor and electrical resistance heating compensation edge temperature control mode, achieving a maximum heating rate of 7℃ and a maximum cooling rate of 5℃, significantly shortening experiment time.

8.  3-second rapid light acquisition mode for the entire plate ensures consistency of experimental results across all wells.

9. Simple and intuitive software guidance makes starting detection experiments easy.

3、Pine wood nematode detector Technical Specifications

1. Features a high-grade aluminum alloy sample holder with excellent thermal conductivity, corrosion resistance, and oxidation resistance, along with Peltier heating and temperature control, and a comprehensive sealing design. Neither sample solution nor condensation will leak into the Peltier temperature control element or other electronic components below the sample holder, effectively extending the instrument's lifespan.

2. Utilizes a high-precision PT1000 temperature sensor, achieving a temperature control accuracy of 0.01℃, ensuring precise temperature control.

3. Sample throughput: 0.2ml single tube, 0.2ml 8-tube strip.

4. Open reagent and consumable system: Compatible with reagents from any brand, suitable for standard 8-tube strips or 0.2ml PCR single tubes.

5. Employs four high-intensity, broad-spectrum, long-life LED solid-state light sources (blue, green, red, and yellow), ensuring strong light intensity across the entire spectral detection range, improving detection sensitivity.  These LEDs generate no heat, have a long lifespan, and are maintenance-free for life.

6. High-sensitivity detector: Uses a high-sensitivity photomultiplier tube imported from Japan for higher detection sensitivity.

7. No optical path correction required: The scanning system ensures that the signal excitation and detection optical paths are completely consistent for each well, eliminating the need for optical path correction. This frees up a channel for correction, enabling true four-channel gene detection. ★

8. Number of channels: 4 channels.

9. Channel crosstalk: Uses high-precision narrow-band filters to greatly reduce channel crosstalk, and the software can also correct crosstalk parameters. 10. Detection Sensitivity: Capable of detecting single-copy DNA templates, and clearly distinguishing between 1000 and 2000 copies of plasmid DNA.

11. Linear Detection Range: 10 orders of magnitude ★

12. Supports common fluorescent dyes and probe detection methods, such as SYBR Green I, Eva Green, Taqman, FRET, Molecular Beacons, Scorpions, Amplifluor, etc.

13. Module Temperature Range: 4-99℃

14. Control Mode: Built-in PCR operating system on the system screen or Windows computer ★

15. Interface: USB connection to computer

16. Operating Noise: Silent technology, maximum operating noise does not exceed 60dB during operation.

17. Operating and Analysis Software

17.1 Allows defining sample wells after running the program, saving experimental time.

17.2 Allows opening other experimental data results for viewing and analysis simultaneously while the program is running.

17.3 The software automatically calculates the baseline range and sets the threshold line, providing users with reliable and accurate data results.

17.4 Experimental program settings and detection results can be directly printed as an experimental report, supporting export in various file formats and data styles such as XLS, CSV, and WORD ★

17.5 Results from the same batch of experiments can be grouped for independent data analysis, with a maximum of 2 groups.

17.6 Provides both Chinese and English software interfaces. ★

17.7 The software can be installed on multiple computers without limitation, requiring no payment for usage rights, and offers free lifetime upgrades. ★

Pine wood nematode detector Instrument technical specifications